Composite

Part:BBa_K3089023:Design

Designed by: Aiai Dong   Group: iGEM19_Greatbay_SCIE   (2019-10-16)


T7 promoter+csgA-linker-mfp5-mfp5-His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal PstI site found at 400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 400
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 585
    Illegal BamHI site found at 813
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal PstI site found at 400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal PstI site found at 400
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Linker to prevent each component jamming together "Strong underwater adhesives made by self-assembling multi-protein nanofibres" (Zhong et al, 2014)


Source

Mfp5 - Mytilus galloprovincialis

References